內(nèi)皮細(xì)胞培養(yǎng)基說明： 內(nèi)皮細(xì)胞培養(yǎng)基是專門為正常人類微血管內(nèi)皮細(xì)胞體外培養(yǎng)設(shè)計(jì)的*適于其生長的完全培養(yǎng)基。是經(jīng)**的液體培養(yǎng)基，包含必需和非必需氨基酸、維生素、有機(jī)和無機(jī)化合物、**、生長因子、微量礦物質(zhì)和低濃度胎牛血清(5%)。該培養(yǎng)基緩沖體系為重碳酸鹽，在含5%CO2的細(xì)胞培養(yǎng)箱中平衡后pH值為7.4。該培養(yǎng)基的配方能夠選擇性的促進(jìn)正常人類微血管內(nèi)皮細(xì)胞體外培養(yǎng)中的增殖和生長，并為其達(dá)到*理想營養(yǎng)平衡狀態(tài)。 內(nèi)皮細(xì)胞培養(yǎng)基包含500 ml基礎(chǔ)培養(yǎng)基，25mll胎牛血清（FBS, Cat. No. 0025） 、5ml 內(nèi)皮細(xì)胞生長因子（ECGS, Cat. No. 1052 ）、5 ml青霉素/鏈霉素溶液(P/S, Cat. No. 0503) 。
ECM說明書
Endothelial Cell Medium(ECM)
Catalog Number: 1001
Product Description
Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human microvascular endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.
Components
ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
Product Use
ECM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at 4℃, the ECGS, the FBS and the P/S solution at -20℃. Protect from light.
Shipping
Gel ice.
Prepare for use
Thaw ECGS, FBS and P/S solution at 37℃. Gently tilt the ECGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ECGS, FBS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37℃. When stored in the dark at 4℃, the reconstituted medium is stable for one month.
Caution:
If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.
